When the alcohol extract was fractionated by sequential solvent extraction, the activity was found in ethyl acetate fraction 50 mgkg. Normal and diabetic control rats received saline, whereas rats in other groups received 300 or 600 mgkg body weight of extract or metformin 100 mgkg body weight of metformin for 6 weeks. Diabetes was induced by interperitoneal injection of alloxan monohydrate 150. Blood glucose level and lipid profile of alloxaninduced. Antihyperglycemic effect was investigated in normal and alloxan induced diabetic rats. View the article pdf and any associated supplements and figures for. It has been successfully induced in a variety of animal species. One alternative therapeutic approach in dm patients is traditional use of herbal medicines such as physalis angulata herbs. Comparative effects of rothmannia hispida leaves extract and. The rats were divided into six groups after the induction of alloxan diabetes.
In alloxan induced diabetic rats by oral administration of extract 100mgkg body weight for 15 days. Diabetes was induced in albino rats by administration of alloxan. There was significant increase in pcv, hb, rbc and wbc in diabetic rats. In this study, oral administration of zanthoxylum chalybeum at different doses for 4 weeks was evaluated for effects of z.
Hypoglycemic effect of polyherbal formulation in alloxan. Diabetes was induced in rats with equal molar dosages of either streptozotocin or alloxan. In this work, it was investigated the effects of diabetogenic doses of alloxan range from 80 to 140 mgkg 80,100,120, and 140 as a single dose by the routes of sc and ip administration. Studies on the administration of vernonia amygdalina del.
The mechanism of alloxan and streptozotocin action in b. In indias indigenous system of medicine, coriandrum sativum cs, commonly used as a food ingredient, is claimed to be useful for various ailments. Effect of dme on alloxan induced diabetic rats a total of twenty five 25 diabetic and five 5 normoglycemic rats of both sexes were used. Dec 18, 2007 alloxan has two distinct pathological effects.
This study evaluated the longterm effects of alloxan induced diabetes in rat liver. Alloxan injection produced severe diabetic renal damage, as revealed by significantly greater malondialdehyde mda levels and superoxide dismutase sod activity in the. Increased oxidative stress and imbalance in antioxidant enzymes in the brains of alloxan induced diabetic rats. Viswanathaswamy, et al antihyperglycemic and antihyperlipidemic activity of plectranthus amboinicus the present study was undertaken to investigate the antihyperglycemic and antihyperlipidemic effects of ethanol extract of plectranthus amboinicus in normal and alloxan induced diabetic rats. The current study sought to investigate the capacities of single and combinatorial herbal formulations of leaf extracts of acanthus montanus, asystasia gangetica, emilia coccinea, and hibiscus rosasinensis to reverse hyperglycemia and dyslipidemia in alloxan induced diabetic male rats. Effects of aqueous extracts of lannea edulis in alloxaninduced diabetic rats. Alloxan was administered as a single dose 120 mgkg bw to induce diabetes. Biochemical and haematological effects of the leaf extract. In the light of the above data, the objectives of the present study were to evaluate the effect of hcq on blood glucose and lipid profile in alloxan induced diabetic rats and to study its effect on the above parameters in com bination with conventional oral antidiabetic agents. This study was therefore designed to establish the relative potency of insulin and r.
Fresh liver weight and its relationship with body weight were. Pdf antidiabetic activity of hf on alloxan induced diabetic rats. Antidiabetic and antioxidant activities of the methanol leaf. Alloxaninduced diabetes mellitus how is alloxaninduced. May 24, 2014 parallel to our result, ghannnam et al. Hypoglycemic activity of cucumis sativus extract on. Sep 30, 2016 in the current investigation, a statistical model for the optimal dose level of alloxan and stz were recognized in order to induce dm in wistar male rats.
Antihyperglycemic and antihyperlipidemic effects of. The aim of the study was to evaluate the anti diabetic effect of isolated compound from p. Antioxidant, antihyperglycemic, and antihyperlipidemic. Fabaceae leaves and flowers on alloxan induced diabetes in rats. Effect of ethanolic extract of pterocarpus marsupium root on. Phytochemicals, hypoglycemic and hypolipidemic effects of. Antidiabetic activity of nerium indicum leaf extract in alloxan. Hypoglycemic and antioxidant effects of leaf essential oil. Pdf antidiabetic activity of extract on alloxan induced diabetic rats. Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Anion gap toxicity in alloxan induced type 2 diabetic rats. This experimental study examined the impact of sildenafil citrate, vitamin a vit a, vitamin c vit c and vitamin e vit c on wound healing in a rat model of diabetes n100. The alloxan model of diabetes was first described in rabbits by dunn, sheehan and mcletchie in 1943.
Streptozotocin use in mice and rats version 1 date of publication. Treatment of diabetic rats with sodium phthalhydrazide partially reversed diabetogenic pathology in the alloxan induced diabetes. Antioxidant and antidiabetic activities of terminalia bellirica fruit in alloxan induced diabetic rats author. The test group was treated with 200 mgkg dosage of moringa oleifera ethanolic leave extract. Pdf diabetes mellitus is affecting many in workforce, thus have a major impact on both individual and national productivity. Alloxan monohydrate is used to induce diabetes mellitus in albino rats in the dose of 120mgkg i. Experimental results also reflect that the tectona grandis is capable of reducing the oxidative state associated with diabetes 25. Antidiabetic effect of ipomoea batatas in normal and alloxan induced diabetic rats. Diabetes was induced in albino rats by administration of alloxan monohydrate 100mgkg,i. In the experiment, six rats were used in each group. Antidiabetic activity of compound isolated from physalis. Alloxan has been administered in single or multiple doses, through different routes. The oral administration of 200, 300, and 400mgkg b. Diabetes in rodents is induced by injecting alloxan or streptozotocin which induce diabetes while producing ros leading to demolition of.
The most plausible explanation for induction of diabetes due to alloxan. Pdf the antidiabetic activity of the hf consisting of three wellknown herbs for diabetes namely, piper betel, butea monosperma and. Antidiabetic activity of nerium indicum leaf extract in. Most studies have shown that alloxan is not toxic to the human betacell, even in very high doses, probably because of differing glucose uptake mechanisms in humans and rodents.
Thirty nondiabetic control rats nc and 30 untreated diabetic ud rats were divided into three subgroups sacrificed after 6, 14, or 26 weeks. Antidiabetic activity of aqueous extract of leptospermum. Hypoglycemic effects in alloxaninduced diabetic rats of the phenolic extract from mongolian oak cups enriched in ellagic acid, kaempferol. Leaf extracts in alloxan induced diabetic rats syed mansoor ahmed, vrushabendra swamy bm, p gopkumar r dhanapal and vm chandrashekara department of pharmacology, m. The extracts of berberis lycium and diabetes mellitus in alloxan monohydrate induced diabetic rats. The clinical course of the diabetes mortality, hyperglycemia, weight loss, polydipsia, hyperphagia, polyuria, glycosuria and diabetic indices was recorded for six weeks before the animals were sacrificed for volumetric quantitation of the pancreatic islets.
The rats randomly selected were divided into six 6 groups of five 5 rats per group. Research article antidiabetic effect of sida cordata in. Alloxan was injected intraperitoneally at a single dose of 150mgkg body weight b. Diabetes was induced by intraperitoneal injection of 120 mgkg b. At the end of the treatment period, blood glucose levels, protein, mda, catalase, glutathione and vitamin c concentrations were determined in both the. Rothmannia hispida herb extract is also used to manage dm by traditional healers.
Rats treated with 100 mgkg bw of methanol leaf extract of hibiscus sabdariffa also exhibited hibiscus sabdariffa. The mechanisms of alloxan and streptozotocin induced diabetes, diabetologia, 2007, pp. Alloxaninduced diabetes, a common model for evaluating. Diabetes was induced in male wistar rats by the administration of alloxan monohydrate at 100 mgkg of body weight. Hemoglobin, packed cell volume, platelets, red blood cells, serum glucose, white blood cells, zingiber officinale. Hypoglycemic and antioxidant effects of leaf essential oil of. Hypoglycemic effect of ginger zingiber officinale in alloxan induced diabetic rats rattus norvagicus 2011. What is the right dose of alloxan to induce diabetes 1 in albino rats. Antidiabetic activity of aqueous extract of sigesbeckia orientalis st. In the in vivo experiment, glucose given to rats 20 min prior to alloxan partially restricted alloxan induced increase in the activity of glutathione peroxidase. Effect of zingiber officinale on some hematologic values. The ethanol extracts of pterocarpus marsupium root was investigated for its antidiabetic effect in wistar albino rats.
Periodontal disease in diabetic patients presents higher severity and prevalence. Effects of aqueous avocado pear persea americana seed. Alloxaninduced diabetes triggers the development of. Dose optimization of alloxan for diabetes in albino mice. Antidiabetic effect of ipomoea batatas in normal and. Diabetes was induced in overnight fasted rats by intraperitoneal injection. Alloxan induced insulin release is, however, of short duration and is followed by complete suppression of the islet response to glucose, even when high concentrations 16. To study the antidiabetic activity of aqueous extract of cinnamomum cassia in alloxan induced diabetic albino rats. Antihyperglycemic effect of gasca d herbal formulation on alloxan.
Alloxan induced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Antidiabetic effect of hydroalcoholic extract of carthamus tinctorius l. Effect of ethanolic extract of pterocarpus marsupium root. The effect of lisinopril on blood glucose level given alone. Significant decrease in body weight also was observed with. To establish its utility in diabetes mellitus, the present study evaluated the antidiabetic and antioxidant effects of cs in alloxan. Original article rat model of foodinduced nonobesetype. The effect of orally administered aqueous extracts 400 mgkg body weight of clitoria ternatea leaves and flowers on serum glucose, glycosylated hemoglobin, and insulin were examined in control and extracttreated diabetic rats. The name is derived from allantoin, a product of uric acid excreted by the fetus into the allantois, and oxaluric acid derived from oxalic acid and urea, found in urine. The effects of dosage and the routes of administrations of. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural. Red kidney beans was investigated for its effects in alloxan induced diabetic rats.
Protective effects of zanthoxylum chalybeum in diabetes. Original article renoprotective effect of grape seed extract. Indeed, in rat islets incubated with alloxan the gsh content and gshgssg ratio were decreased malaisse et al. The mechanisms of alloxan and streptozotocininduced. Antidiabetic effect of sida cordata in alloxan induced diabetic rats. In 1943, alloxan became of interest in diabetes research when dunn and mcletchie reported that it could induce diabetes in animals 4 as a result of the specific necrosis of the pancreatic beta cells 57. Marked degenerations of the islets of langerhans was observed in pancreas of alloxan induced diabetic rats, whereas, histological examination of the pancreas of. The mechanisms of alloxan and streptozotocininduced diabetes. Alloxan is a urea derivative which causes selective necrosis of the. An alloxaninduced diabetic mice test model was used. The prime focus of this study is to estimate the potential of ipomoea batatas l peeloff on magnesium and calcium level in alloxan induced diabetic rats. Hypoglycemic effect of ginger zingiber officinale in alloxan. Dose optimization of alloxan for diabetes in albin. Vitamin c and vitamin e on wound healing in alloxaninduced diabetic rats a tas1, a karasu1, z yener2, s y.
Antidiabetic activity of aqueous extract of cinnamomum. Indeed, the essential oil of rosescented geranium leaves was prepared and their antioxidant activities as well as their hypoglycemic effects were studied. Induction of diabetes mellitus alloxan monohydrate was used to induce diabetes mellitus. Furthermore, alloxan inhibits glucokinase, a shcontaining protein essential for insulin secretion induced by glucose. Alloxan monohydrate was used to induce diabetes in the rats. Group 3 diabetic rats that were given leucas lavandulaefolia.
The alloxan treated rats with permanent hyperglycemia, which further received i. The animals were distributed into 4 groups of 5 rats each namely. Diabetes was induced in rats two weeks before starting the experiment. Hypoglycemic effect of leucas lavandulaefolia willd in. Antioxidant and antidiabetic activities of terminalia. The aqueous extract of cinnamon 60mgkg was studied alone and in combination with conventional oral antidiabetic drugs like glibenclamide 5mgkg and metformin 0. Alloxan definition of alloxan by medical dictionary. Antihyperglycemic and antihyperlipidemic activity of.
Partial recovery from alloxaninduced diabetes by sodium. Comparison of streptozotocin and alloxaninduced diabetes in. It is used as a cleansing and purifying agent and yet can be considered as a bodybuilder because of its nutritional value. The experimental animals were grouped into five groups ae of 10 animals each. Rats treated with 7% protein isolate recorded the most significant p diabetic and hypoglycaemic effects on alloxan induced diabetic rats. Hence, the present study undertakes the investigation of possible hypoglycemic and antioxidant effects of pelargonium graveolens leaves in alloxan induced diabetic rats. Wheatgrass has many characteristics desirable for health. To understand the pathophysiological mechanisms and factors involved in diabetes, the use of pancreatic regeneration studies is increasing in an attempt to understand the behavior of pancreatic beta cells. It was observed that in normal rats the lisinopril slightly reduced the blood glucose level at all time points which was significant only at 30 and 120 min time points p. Antidiabetic activity of ethanol extract of colocasia esculenta leaves in alloxan induced diabetic rats. Original article rat model of foodinduced nonobesetype 2. This fraction containing steroids and coumarins showed antidiabetes activity in alloxan diabetic rats as judged from serum glucose levels, liver glycogen content and body weight.
In this study, diabetic mice were given 10 mgkg and 500 mgkg of glibenclamide and aloe, respectively, twice per day for 14 days. Antidiabetic and hypolipidaemic properties of ginger. This research aims to investigate the antidiabetic activity of nerium indicum family. Apocynaceae leaf extract in alloxan induced diabetic albino rats. Hypoglycemic activity of cucumis sativus extract on alloxan. The extract improve the liver, kidney and pancreas function and reduce lesions associated with diabetic st ate in alloxan induced rats. Antidiabetic activity of alcoholic extract of leaves of colocsia. Research article alloxan induced diabetes mellitus in. The present study was carried out to investigate the effects of onion allium cepa linn and garlic allium sativum linn juices on biochemical parameters, enzyme activities and lipid peroxidation in alloxan induced diabetic rats. The mechanism of alloxan and streptozotocin action in b cells. What is the right dose of alloxan to induce diabetes 1 in. Wound healing activity of ethanolic extract of allium sativum on alloxan induced diabetic rats family liliaceae mohammad. Rats with blood glucose levels above 250 mgdl were considered diabetic 30 and selected for the study. The blood sugar and body weight of the rats was recorded at two weeks and four weeks interval, and one week after the withdrawal of the extract.
The present study was carried out to evaluate the antidiabetic effect of psidium guajava leaves extract and to study the activities of hexokinase, aldolase and phosphor glucoisomerase, and gluconeogenic enzymes such asglucose6phosphatase and. However, in absence of aggressive stimuli such as ligatures, the influence of diabetes on rat periodontal tissues is incompletely explored. The treatment of alloxan induced diabetic rats with the tgm extract and significantly p diabetic group. Analysis of certain biochemical indices on alloxan induced. The induction of diabetes was confirmed after the 5th day of alloxan treatment by. Antidiabetic effect of ipomoea batatas in normal and alloxan. This work evaluated the effects of different doses of the human urine on normal and alloxan induced diabetic rats. In the alloxan induced diabetic rats showed that extract at 500 mgkg decreased significantly fasting plasma glucose level by 61. The rats are divided into 6 groups and each group con tains 6 rats n 6 as follows. Tirupathi mallareddy college of pharmacy, dundigal, rangareddy, andhra pradesh, india. Twenty four albino rats were randomly allocated into four groups ad of six rats each such that group a diabetes control received 0. Antidiabetic drugs in combination with hydroxychloroquine. Alloxan diabetes was induced into 20 rats and the rats weighed 125g. Antidiabetic effect of hydroalcoholic extract of carthamus.
The alloxan induced diabetic rats were grouped into three groups containing at least 5 rats in each group. Alloxan induced diabetic untreated rats showed a progressive decrease body weight figure 2 with final body weight loss of 32. In research it is used for induction of type 1 diabetes. Please see the supplementary file for standards hplc chromatograms figure s1. Blood glucose lowering activity of aloe based composition. Sensitization to alloxan induced diabetes and pancreatic cell apopto. The experimental design comprises of five 5 groups of four 4 animals each. Anti diabetic and hypolipidaemic properties of ginger zingiber officinale in streptozotocin induced diabetic rats volume 96 issue 4 zainab m.
Diabetes induced by coxsackie virus infection is a direct. Hypoglycemic effects in alloxaninduced diabetic rats of the. E, and ceruloplasmin in streptozotocininduced diabetic rats. After 48 h, rats with fasting blood glucose levels of 200 mgdl and above were considered diabetic and used for the study. Hypoglycemic effects of aeso in alloxaninduced diabetic rats. Fresh leaves of the plant were collected from their local habitat in the university of nigeria, nsukka environment.
Hypoglycaemic activity and nephroprotectective effect of. Effects of the aqueous extract on groups of alloxan 150mgkg induced diabetic rats was investigated. Marked degenerations of the islets of langer hans was observed in pancreas of alloxaninduced diabetic rats, whereas, histological examination. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22. Oral administration of polyherbal formulation to diabetic animals up to four weeks dose dependently reduced the blood glucose level, which was comparable to that of glibenclamide 5 mgkg. Research article antidiabetic effect of sida cordata in alloxan induced diabetic rats naseeralishahandmuhammadrashidkhan department of biochemistry, faculty of. Jun 26, 2012 hence, the present study undertakes the investigation of possible hypoglycemic and antioxidant effects of pelargonium graveolens leaves in alloxan induced diabetic rats. Antidiabetic activity of leaves of jatropha curcus l.
1018 735 621 1018 1444 435 240 515 236 346 1058 564 1143 1489 1180 1107 323 813 1083 1388 126 1125 136 1110 790 1236 170 846 70 266 902